Difference between revisions of "Main Page"
From Leschziner public wiki
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In these pages you will find tutorials designed to explain, in an accessible way, some of the principles behind the techniques used in our lab. They range from the very general (How does a microscope work?) to very specific approaches used in Three-Dimensional Electron Microscopy. This wiki is, by definition, a "work in progress"; we will periodically add new material and update what's already here. <span style="color:#14a;">Blue</span> links are for tutorials already available while <span style="color:#ff0000">red</span> ones are for those we are still developing. | In these pages you will find tutorials designed to explain, in an accessible way, some of the principles behind the techniques used in our lab. They range from the very general (How does a microscope work?) to very specific approaches used in Three-Dimensional Electron Microscopy. This wiki is, by definition, a "work in progress"; we will periodically add new material and update what's already here. <span style="color:#14a;">Blue</span> links are for tutorials already available while <span style="color:#ff0000">red</span> ones are for those we are still developing. | ||
| − | We hope you find the material useful and welcome feedback on how to improve it. To give us feedback, please see the [[Talk:Main_Page|discussion tab]] at the top of the page. | + | We hope you find the material useful and welcome feedback on how to improve it. To give us feedback, please see the [[Talk:Main_Page|discussion tab]] at the top of the page, or [mailto:leschziner.tutorials@gmail.com e-mail us]. |
==General imaging principles== | ==General imaging principles== | ||
| + | These conventions and basic physics concepts are fundamental tools for understanding electron imaging. | ||
*[[Light optics]] | *[[Light optics]] | ||
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==Electron imaging== | ==Electron imaging== | ||
| − | + | There are many caveats to imaging with electrons as opposed to photons. | |
| − | There are many | ||
*[[What is an Electron Microscope?]] | *[[What is an Electron Microscope?]] | ||
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*[[Types of Signals]] | *[[Types of Signals]] | ||
*[[Electron optics]] | *[[Electron optics]] | ||
| + | *[[The Weak-Phase Object Approximation]] | ||
*[[Contrast]] | *[[Contrast]] | ||
*[[Ewald Sphere]] | *[[Ewald Sphere]] | ||
| − | *[[ | + | *[[Special Imaging Techniques]] |
| − | |||
| − | == | + | ==Electron Microscopy of Macromolecules== |
*[[Sample Preparation]] | *[[Sample Preparation]] | ||
| + | *[[Special Considerations]] | ||
| + | |||
| + | ==Two-Dimensional Averaging== | ||
==Three-dimensional reconstruction by EM== | ==Three-dimensional reconstruction by EM== | ||
| + | |||
A "30 Second Overview" of three-dimensional EM reconstruction. | A "30 Second Overview" of three-dimensional EM reconstruction. | ||
<qt>file=EM intro2.mov|width=768|height=576|autoplay=false|controller=true</qt> | <qt>file=EM intro2.mov|width=768|height=576|autoplay=false|controller=true</qt> | ||
| + | ===Basics=== | ||
| + | *[[Central Section Theorem]] | ||
| + | *[[Back Projection]] | ||
| − | ===Reconstruction Methods=== | + | ===Existing Reconstruction Methods=== |
*[[Angular Reconstitution]] (AR) | *[[Angular Reconstitution]] (AR) | ||
*[[Random Conical Tilt]] (RCT) | *[[Random Conical Tilt]] (RCT) | ||
*[[Orthogonal Tilt Reconstruction]] (OTR) | *[[Orthogonal Tilt Reconstruction]] (OTR) | ||
| − | === | + | ===Software Available=== |
| + | An overview of the currently available software, links, and general descriptions. | ||
| + | |||
| + | ==Resolution== | ||
| + | |||
| + | ==Hardware Innovations to the Electron Microscope== | ||
Some new, some old, there are many aspects of the microscope that may be changed, ostensibly altering the physics of image formation for the better. | Some new, some old, there are many aspects of the microscope that may be changed, ostensibly altering the physics of image formation for the better. | ||
*[[CMOS]] | *[[CMOS]] | ||
*[[Phase Plates]] | *[[Phase Plates]] | ||
| − | |||
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| − | |||
| − | |||
| − | |||
| − | |||
---- | ---- | ||
Back to [https://leschzinerlab.ucsd.edu/ Leschziner Lab Main Site] | Back to [https://leschzinerlab.ucsd.edu/ Leschziner Lab Main Site] | ||
Revision as of 20:46, 30 April 2010
In these pages you will find tutorials designed to explain, in an accessible way, some of the principles behind the techniques used in our lab. They range from the very general (How does a microscope work?) to very specific approaches used in Three-Dimensional Electron Microscopy. This wiki is, by definition, a "work in progress"; we will periodically add new material and update what's already here. Blue links are for tutorials already available while red ones are for those we are still developing.
We hope you find the material useful and welcome feedback on how to improve it. To give us feedback, please see the discussion tab at the top of the page, or e-mail us.
Contents
General imaging principles
These conventions and basic physics concepts are fundamental tools for understanding electron imaging.
Electron imaging
There are many caveats to imaging with electrons as opposed to photons.
- What is an Electron Microscope?
- History of the Electron Microscope
- Types of Signals
- Electron optics
- The Weak-Phase Object Approximation
- Contrast
- Ewald Sphere
- Special Imaging Techniques
Electron Microscopy of Macromolecules
Two-Dimensional Averaging
Three-dimensional reconstruction by EM
A "30 Second Overview" of three-dimensional EM reconstruction.
Basics
Existing Reconstruction Methods
- Angular Reconstitution (AR)
- Random Conical Tilt (RCT)
- Orthogonal Tilt Reconstruction (OTR)
Software Available
An overview of the currently available software, links, and general descriptions.
Resolution
Hardware Innovations to the Electron Microscope
Some new, some old, there are many aspects of the microscope that may be changed, ostensibly altering the physics of image formation for the better.
Back to Leschziner Lab Main Site
