Difference between revisions of "Sample Preparation"

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(Created page with 'Intro ==Support Grids== ==Negative Stain== ===Artifacts=== ==Vitreous Ice Embedment== ===Cryo-Negative Staining=== ==Labeling with Gold Clusters==')
 
 
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Intro
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Imaging biological samples in their native state in the electron microscope can see like an impossible task: how can one immobilize a protein instantly and place it into the extreme environment of a vacuum without any structural changes occurring? However, advances in sample preparation techniques over the years have made it possible to do practically that.
  
 
==Support Grids==
 
==Support Grids==

Latest revision as of 18:00, 7 May 2010

Imaging biological samples in their native state in the electron microscope can see like an impossible task: how can one immobilize a protein instantly and place it into the extreme environment of a vacuum without any structural changes occurring? However, advances in sample preparation techniques over the years have made it possible to do practically that.

Support Grids

Negative Stain

Artifacts

Vitreous Ice Embedment

Cryo-Negative Staining

Labeling with Gold Clusters