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Welcome to the Leschziner Lab's Tutorials Wiki

In these pages you will find tutorials designed to explain, in an accessible way, some of the principles behind the techniques used in our lab. They range from the very general (How does a microscope work?) to very specific approaches used in Three-Dimensional Electron Microscopy. This wiki is, by definition, a "work in progress"; we will periodically add new material and update what's already here. Blue links are for tutorials already available while red ones are for those we are still developing.

We hope you find the material useful and welcome feedback on how to improve it. To give us feedback, please see the discussion tab at the top of the page.

General imaging principles

• Light optics
• Microscope Ray Tracing
• Phase Contrast
• Depth of Field

Electron imaging

There are many topics to take into account when imaging with electrons.

• What is an Electron Microscope?
• History of the Electron Microscope
• Types of Signals
• Electron optics
• Contrast
• Ewald Sphere
• Central Section Theorem
• Back Projection

Three-dimensional reconstruction by EM

A "30 Second Overview" of three-dimensional EM reconstruction.

Reconstruction Methods

• Angular Reconstitution (AR)
• Random Conical Tilt (RCT)
• Orthogonal Tilt Reconstruction (OTR)

Accessories to the Electron Microscope

Some new, some old, there are many aspects of the microscope that may be changed, ostensibly altering the physics of image formation for the better.

• CMOS
• Phase Plates

Software Available

An overview of the currently available software, links, and general descriptions.

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