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Leschziner Lab Tutorials

The following tutorials are designed to aid in the understanding of the physical principles associated with our lab's research. Our goal is to make these principles accessible to all scientists, so that we may all participate in informed discourse. Once edited and polished on our lab wiki's testing environment, these tutorials are freely available to the public.

Note: This initial offering is a sample of the topics that we will be publishing on. Examples are available where there are blue links, while red links indicate pages not yet created.

General imaging principles

• Light optics

Electron imaging

There are many considerations in imaging with electrons.

• Electron optics
• Contrast
• Ewald Sphere
• Central Section Theorem

Three-dimensional reconstruction by EM

A "30 Second Overview" of three-dimensional EM reconstruction.

File:EM intro2.mov

Reconstruction Methods

• Angular Reconstitution (AR)
• Random Conical Tilt (RCT)
• Orthogonal Tilt Reconstruction (OTR)

Accessories to the Electron Microscope

Some new, some old, there are many aspects of the microscope that may be changed, ostensibly altering the physics of image formation for the better.

• CMOS
• Phase Plates

Software Available

An overview of the currently available software, links, and general descriptions.

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