Main Page - Revision history

Bpappas at 18:13, 7 May 2010

2010-05-07T18:13:31Z

Bpappas: /* Electron Microscopy of Macromolecules */

2010-05-07T18:00:29Z

‎Electron Microscopy of Macromolecules

Bpappas: /* Two-Dimensional Averaging */

2010-05-07T17:59:35Z

‎Two-Dimensional Averaging

Bpappas: /* Electron Microscopy of Macromolecules */

2010-05-07T17:57:40Z

‎Electron Microscopy of Macromolecules

Bpappas: /* Electron Microscopy of Macromolecules */

2010-05-07T17:56:49Z

‎Electron Microscopy of Macromolecules

Bpappas: /* General imaging principles */

2010-05-07T17:48:38Z

‎General imaging principles

Bpappas: /* Introduction */

2010-05-07T17:47:43Z

‎Introduction

Bpappas: /* Introduction */

2010-05-07T17:33:05Z

‎Introduction

Bpappas: /* Introduction */

2010-05-07T16:48:13Z

‎Introduction

Bpappas at 16:42, 7 May 2010

2010-05-07T16:42:14Z

← Older revision		Revision as of 18:13, 7 May 2010
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	In these pages you will find tutorials designed to explain, in an accessible way, some of the principles behind the techniques used in our lab. They range from the very general (How does a microscope work?) to very specific approaches used in Three-Dimensional Electron Microscopy (3D-EM). This wiki is, by definition, a "work in progress"; we will periodically add new material and update what's already here. <span style="color:#14a;">Blue</span> links are for tutorials already available while <span style="color:#ff0000">red</span> ones are for those we are still developing.		In these pages you will find tutorials designed to explain, in an accessible way, some of the principles behind the techniques used in our lab. They range from the very general (How does a microscope work?) to very specific approaches used in Three-Dimensional Electron Microscopy (3D-EM). This wiki is, by definition, a "work in progress"; we will periodically add new material and update what's already here. <span style="color:#14a;">Blue</span> links are for tutorials already available while <span style="color:#ff0000">red</span> ones are for those we are still developing.
		+
		+	In the Leschziner Lab, we use electron microscopy to study structures of macromolecules. In particular, we look at single particles and use the techniques described in the sections below to generate three-dimensional structures of the proteins or protein complexes of interest. In addition to the study of protein structure, our lab is interested in improving techniques for 3D-EM.

	We hope you find the material useful and welcome feedback on how to improve it. To give us feedback, please see the [[Talk:Main_Page\|discussion tab]] at the top of the page, or [mailto:leschziner.tutorials@gmail.com e-mail us].		We hope you find the material useful and welcome feedback on how to improve it. To give us feedback, please see the [[Talk:Main_Page\|discussion tab]] at the top of the page, or [mailto:leschziner.tutorials@gmail.com e-mail us].

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 ==Electron Microscopy of Macromolecules==
-Imaging biological samples in the electron microscope can see like an impossible task: how can one immobilize a protein instantly and place it into the extreme environment of a vacuum without any structural changes occurring? However, advances in sample preparation techniques over the years have made it possible to do practically that.
+(Pipeline Picture)
 *[[Sample Preparation]]

← Older revision		Revision as of 17:59, 7 May 2010
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	==Two-Dimensional Averaging==		==Two-Dimensional Averaging==

		+	Once the data from imaging the macromolecules of interest are collected, several steps are taken to sift through the images and create a three-dimensional model.

	==Three-dimensional reconstruction by EM==		==Three-dimensional reconstruction by EM==

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 *[[Sample Preparation]]
 *[[Special Considerations]]
 ==Two-Dimensional Averaging==

← Older revision		Revision as of 17:56, 7 May 2010
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	==Electron Microscopy of Macromolecules==		==Electron Microscopy of Macromolecules==
		+
		+	Imaging biological samples in the electron microscope can see like an impossible task: how can one immobilize a protein instantly and place it into the extreme environment of a vacuum without any structural changes occurring? However, advances in sample preparation techniques over the years have made it possible to do practically that.

	*[[Sample Preparation]]		*[[Sample Preparation]]

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 ==General imaging principles==
-These conventions and basic physics concepts are fundamental tools for understanding electron imaging.
+These conventions and basic physics concepts are fundamental tools for understanding optical and electron imaging.
 *[[Light optics]]

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 ==Introduction==
-The compound optical microscope, first recognized in 1590, is probably the most familiar of all laboratory instruments, especially for biologists. The electron microscope, for many years simply an exotic rarity, has also become a standard tool in biological research. Both instruments are often used quite effectively with little knowledge of the relevant theory, or even how the particular microscope functions. Eventually, however, proper use, interpretation of images, and choices of specific application demand an understanding of fundamental principles.
+The compound optical microscope, first recognized in 1590, is perhaps the most familiar of all laboratory instruments. The much younger electron microscope, for many years simply an exotic rarity, has also become a standard tool in biological research. As the convenience and accessibility of each has grown over the years, however, the understanding of relevant theory or even how the microscope itself functions has been lost among many users. While a firm understanding of the fundamental principles of optics and imaging is not often necessary for general function, eventually this knowledge becomes necessary for the proper use, interpretation of images, and choices of specific application. This is especially relevant for those who wish to innovate, or even effectively trouble-shoot, in the field.
-This wiki is intended to provide both a brief overview of such concepts, as well as a more in-depth theoretical view for those readers who would like to examine more of the mathematics and physics behind imaging (such as with image formation, contrast, resolution limits). In particular, Fourier imaging will be discussed as it applies to the interpretation of data.
+This wiki is intended to provide both a brief overview of the fundamental concepts, as well as a more in-depth theoretical view for those readers who would like to examine more of the mathematics and physics behind imaging (such as with image formation, contrast, resolution limits). In particular, Fourier imaging will be discussed as it applies to the interpretation of data.
 Since our focus is not directed toward crystalline data sets but rather what can be referred to as asymmetric structures, the image processing and data interpretation sections will deal with techniques particular to the latter.

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 <center><big> '''Welcome to the Leschziner Lab's Tutorials Wiki''' </big></center>
-In these pages you will find tutorials designed to explain, in an accessible way, some of the principles behind the techniques used in our lab. They range from the very general (How does a microscope work?) to very specific approaches used in Three-Dimensional Electron Microscopy. This wiki is, by definition, a "work in progress"; we will periodically add new material and update what's already here. <span style="color:#14a;">Blue</span> links are for tutorials already available while <span style="color:#ff0000">red</span> ones are for those we are still developing.
+In these pages you will find tutorials designed to explain, in an accessible way, some of the principles behind the techniques used in our lab. They range from the very general (How does a microscope work?) to very specific approaches used in Three-Dimensional Electron Microscopy (3D-EM). This wiki is, by definition, a "work in progress"; we will periodically add new material and update what's already here. <span style="color:#14a;">Blue</span> links are for tutorials already available while <span style="color:#ff0000">red</span> ones are for those we are still developing.
 We hope you find the material useful and welcome feedback on how to improve it. To give us feedback, please see the [[Talk:Main_Page|discussion tab]] at the top of the page, or [mailto:leschziner.tutorials@gmail.com e-mail us].
 ==General imaging principles==
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 ==Resolution==
 ==Hardware Innovations to the Electron Microscope==